Identification of several high-risk HPV inhibitors and drug targets with a novel high-throughput screening assay

Author summary Human papillomaviruses are causative agents of many different cancers; they are most commonly associated with cervical cancer which leads to about quarter of a million deaths each year. Regardless of extensive studies for decades there is no specific cure against HPV infection. During this research, we have engineered modified HPV marker genomes that express Renilla luciferase reporter gene which expression level correlates directly with viral genome copy number. We have used such modified HPV genome in high-throughput screening of NCI Diversity Set IV chemical library and have identified a number of novel high-risk HPV-specific chemical compounds and drug targets. Such Renilla-expressing marker genomes could be used in various cell systems suitable for HPV replication studies to conduct high-throughput screens and quantify viral genome copy number quickly and effectively.See it on Scoop.it, via Viruses and Bioinformatics from Virology.uvic.ca
Identification of several high-risk HPV inhibitors and drug targets with a novel high-throughput screening assay
Source: Viral Bioinformatics