Misunderstood parameter of NCBI BLAST impacts the correctness of bioinformatics workflows
“BLAST remains the tool of choice for many use cases, and continues to be actively used in many bioinformatics workflows. Despite the tremendous collective experience of the bioinformatics community with BLAST, the full functionality of this tool remains poorly understood. Here we report on a feature of BLAST that operates in a non-intuitive way and that is frequently misused in bioinformatics workflows, potentially leading to erroneous results impacting numerous scientific articles.”
Source: Viral Bioinformatics
